Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Peptide Nucleic Acids: Methods and Protocols, Second Edition serves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.

Inhaltsangabe1. MiniPEG-gPNAArunava Manna, Srinivas Rapireddy, Raman Bahal, and Danith H. Ly 2. Cyclopentane Peptide Nucleic AcidsEthan A. Englund, Ning Zhang, Daniel H. Appella 3. Chiral PNAs with Constrained Open-chain BackbonesRoberto Corradini, Tullia Tedeschi, Stefano Sforza and Rosangela Marchelli 4. Synthesis, Characterization and Evaluation of Radiometal-Containing Peptide Nucleic AcidsHolger Stephan, Christian Förster, and Gilles Gasser 5. Preparation of Metal-Containing Peptide Nucleic Acid Bioconjugates on the Solid PhaseGilles Gasser 6. Formation and Characterization of PNA-Containing HeteroquadruplexesBruce A. Armitage 7. Sequence Selective Recognition of Double Stranded RNA using Triple Helix-forming Peptide Nucleic AcidsThomas Zengeya, Pankaj Gupta, and Eriks Rozners 8. Assembly of PNA-tagged Small Molecules, Peptides and Carbohydrates onto DNA Templates: Programming the  Combinatorial Pairing and Inter-ligand DistanceNicolas Winssinger, Katarzyna Gorska, Mihai Ciobanu, Jean Pierre Daguer, and Sofia Barluenga 9. Site-selective Scission of Human Genome using PNA-based Artificial Restriction DNA CutterKenichiro Ito and Makoto Komiyama 10. PNA Openers and their Applications for Bacterial DNA DiagnosticsIrina V. Smolina  and Maxim D. Frank-Kamenetskii  11. DNA-templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids - A Versatile Tool for Single Base Specific Detection of Nucleic AcidsAlexander Roloff, Simon Ficht, Christian Dose, and Oliver Seitz 12. Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface MethodsStefano Sforza, Tullia Tedeschi, Mariangela Bencivenni, Alessandro Tonelli, Roberto Corradini, and Rosangela Marchelli 13. Analysis of PNA Hybridization by Surface Plasmon ResonanceBruce A. Armitage 14. PNA Fluorescent in situ Hybridization (FISH) For Rapid Microbiology and Cytogenetic AnalysisHenrik Stender, Brett William, and James Coull 15. Rapid miRNA Imaging in Cells using Fluorogenic Templated Staudinger Reaction Between PNA-based ProbesKatarzyna Gorska and Nicolas Winssinger 16. Cellular Delivery of Peptide Nucleic Acids (PNAs) Takehiko Shiraishi and Peter E. Nielsen 17. Peptide Nucleic Acid Mediated Recombination For Targeted Genomic Repair and ModificationErica B. Schleifman and Peter M. Glazer 18. Antisense Effects of PNAs in BacteriaShan Goh, Jem Stach, and Liam Good